![[RP1100] ExcelRT™一步法RT-PCR試劑盒, 50 RXN](/web/image/product.product/269/image_1024/%5BRP1100%5D%20%5BRP1100%5D%20ExcelRT%E2%84%A2%E4%B8%80%E6%AD%A5%E6%B3%95RT-PCR%E8%A9%A6%E5%8A%91%E7%9B%92%2C%2050%20RXN?unique=0527691)
Description
The ExcelRT™ One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The ExcelRT™ One-Step RT-PCR Kit provides the user an alternative to the lengthy two step processes (first strand generation and amplification) by using a single mixture, single tube, one step reaction. The ExcelRT™ One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+ and enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRT™ One-Step RT-PCR Kit allows the user to complete the RT-PCR process using a thermocycler in a single reaction setting. The ExcelRT™ One-Step RT-PCR Kit is capable of detecting even trace amounts of target RNA and ideal for target RNA amplification and analysis.
Features
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Generates dsDNA directly from RNA samples
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High sensitivity and yields
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High reproducibility, reduced pipetting errors
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High throughput
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Reduced RNase H ribonuclease activity
Storage
-20°C for 24 months
Contents
Storage -20°C for 24 months |
High sensitivity and yields
Sara Moradi, Mohamadreza Alivand, Yaser KhajeBishak, Mohamad AsghariJafarabadi, Maedeh Alipour, Amirhosien Faghfouri, Beitullah Alipour, Int J Surg Protoc . 2021 Aug 19;25(1):184-193. doi: 10.29337/ijsp.161. eCollection 2021.
PMCID: PMC8378073
Sara Moradi, Mohamadreza Alivand, Yaser KhajeBishak, Mohamad AsghariJafarabadi, Maedeh Alipour, Philip D. Chilibeck, and Beitullah Alipourcorresponding author, BMC Sports Sci Med Rehabil. 2021; 13: 48. Published online 2021 May 8. doi: 10.1186/s13102-021-00266-4
PMCID: PMC8106165
[RP1000] ExcelRT™ Reverse Transcriptase
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High yield
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Thermostable, up to 50°C, during first strand synthesis
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High processivity, generating cDNA up to 8 kb
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Reduced RNase H ribonuclease activity
[NS1000] FluoroVue™ Nucleic Acid Gel Stain
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Excellent for in-gel staining
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Sensitivity up to 0.14 ng DNA or 1 ng total RNA
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A safer alternative to EtBr
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Suitable to blue or UV light
Excellent for in-gel staining
Sensitivity up to 0.14 ng DNA or 1 ng total RNA
A safer alternative to EtBr
Suitable to blue or UV light
[RQ2110] ExcelRT™ 2X One-Step RT-qPCR Kit (TaqMan, ROX)
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High yield
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Reverse transcription at wide temperature range (42-60°C)
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High specificity
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Suitable for fast cycle program
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With ROX reference dye