![[TP1000] ExcelTaq™ Taq DNA聚合酶, 5 U/μl, 500 U](/web/image/product.product/316/image_1024/%5BTP1000%5D%20%5BTP1000%5D%20ExcelTaq%E2%84%A2%20Taq%20DNA%E8%81%9A%E5%90%88%E9%85%B6%2C%205%20U-%CE%BCl%2C%20500%20U?unique=0527691)
![[TP1000] ExcelTaq™ Taq DNA聚合酶, 5 U/μl, 500 U](/web/image/product.product/316/image_128/%5BTP1000%5D%20%5BTP1000%5D%20ExcelTaq%E2%84%A2%20Taq%20DNA%E8%81%9A%E5%90%88%E9%85%B6%2C%205%20U-%CE%BCl%2C%20500%20U?unique=0527691)
Description
ExcelTaq™ Taq DNA Polymerase is a recombinant thermo-stable Taq DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With a high DNA synthesis rate and high thermo-stability, ExcelTaq™ Taq DNA Polymerase is suitable for common and specialized PCR applications.
Features
-
5'→3' DNA polymerase activity
-
5'→3' exonuclease activity
-
No detectable 3'→5' exonuclease (proofreading) activity
-
Generates PCR products with 3’-dA overhangs
-
Thermo-stable – half-life lasts for more than 40 min at 95°C
Applications
-
Routine PCR
-
Amplification of DNA fragments up to 8 kb
-
Generation of PCR products for TA cloning
-
DNA labeling
Storage
-20°C for 24 months
Elongation capability
ExcelTaq™ Taq DNA Polymerase can amplify PCR products from λDNA up to 15 kb (M: DM3100).
Sensitivity
ExcelTaq™ Taq DNA Polymerase can amplify PCR products from as little as 1 pg of template DNA (M: DM3100).
Contents
|
Storage Buffer
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol
10X Taq buffer
200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgCl2, 1% Triton X-100
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage
-20°C for 24 months
Recommended PCR Condition
|
Template |
1 – 150 ng |
|
Forward primer |
0.1 – 0.5 µM |
|
Reverse primer |
0.1 – 0.5 µM |
|
10X Taq Buffer |
5 µl |
|
dNTPs |
0.2 mM (each) |
|
Taq DNA Polymerase |
0.25 µl (1.25U) |
|
H2O |
to 50 µl |
|
Total volume |
50 µl |
Recommended PCR Program
|
Steps |
Temp. |
Time |
Cycles |
|
Template denature |
94 °C |
2 min |
1 |
|
Denature |
94 °C |
30 sec |
25-40 |
|
Annealing |
50-68 °C * |
30 sec |
|
|
Extension |
72 °C |
30 sec/kb |
|
|
Final extension |
72 °C |
1 min |
1 |
* Optimal PCR condition varies according to primers’ thermodynamic properties.
The inhibitory effects of PGG and EGCG against the SARS-CoV-2 3C-like protease
PMCID: PMC7787066
PMCID: PMC6420622
[RP1000] ExcelRT™ Reverse Transcriptase
-
High yield
-
Thermostable, up to 50°C, during first strand synthesis
-
High processivity, generating cDNA up to 8 kb
-
Reduced RNase H ribonuclease activity
[TP5000] ExcelTaq™ Hot Start II DNA Polymerase
-
Aptamer-based hot start PCR
-
Reversible enzyme inactivation
-
Omits extra enzyme activation step
-
Convenient for room temperature PCR set-up
-
High yield and specificity of target amplicons
-
Wide range of amplicon length (up to 10 kb)
-
High sensitivity (as low as 1 fg of plasmid)
[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)
-
High Stability
-
Fast Hot Start
-
High Sensitivity
-
Low Background / High Specificity
-
Suitable for Fast Program
-
Smart Blue Contrast Dye
[TP1200] ExcelTaq™ 5X PCR Master Dye Mix
-
5’→3’ DNA polymerase activity
-
No detectable 3'→5' exonuclease (proofreading) activity
-
Generates PCR products with 3'-dA overhangs
-
High yield PCR
-
High reproducibility
-
Reduced pipetting errors
-
Includes tracking dye for direct loading after PCR